RNA as a Flexible Scaffold for Proteins: Yeast Telomerase and Beyond
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چکیده
منابع مشابه
RNA as a flexible scaffold for proteins: yeast telomerase and beyond.
Yeast telomerase, the enzyme that adds a repeated DNA sequence to the ends of the chromosomes, consists of a 1157- nucleotide RNA (TLC1) plus several protein subunits: the telomerase reverse transcriptase Est2p, the regulatory subunit Est1p, the nonhomologous end-joining heterodimer Ku, and the seven Sm proteins involved in ribonucleoprotein (RNP) maturation. The RNA subunit provides the templa...
متن کاملYeast telomerase RNA: a flexible scaffold for protein subunits.
In the yeast Saccharomyces cerevisiae, distinct regions of the 1.2-kb telomerase RNA (TLC1) bind to the catalytic subunit Est2p and to accessory proteins. In particular, a bulged stem structure binds the essential regulatory subunit Est1p. We now show that the Est1p-binding domain of the RNA can be moved to three distant locations with retention of telomerase function in vivo. We present the Es...
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Determination of the structure of the yeast telomerase RNA component TLC1 has been hampered by its large size and high rate of evolutionary divergence. But detailed phylogenetic comparisons have now revealed the unusually flexible and modular architecture of this important RNA molecule.
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The number of essential telomerase components in the active ribonucleoprotein (RNP) has important implications for its mechanism of action yet is by and large unknown. We report that two differentially tagged TLC1 RNAs endogenously expressed in a heterozygous diploid and simultaneously detected via multi-color fluorescence in situ hybridization (FISH) experiments do not co-localize. Probabilist...
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BACKGROUND Telomerase is a ribonucleoprotein complex whose RNA moiety dictates the addition of specific simple sequences onto chromosomes ends. While relevant for certain human genetic diseases, the contribution of the essential telomerase RNA to RNP assembly still remains unclear. Phylogenetic analyses of vertebrate and ciliate telomerase RNAs revealed conserved elements that potentially organ...
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ژورنال
عنوان ژورنال: Cold Spring Harbor Symposia on Quantitative Biology
سال: 2006
ISSN: 0091-7451,1943-4456
DOI: 10.1101/sqb.2006.71.011